Baculovirus

By inserting the gene of interest into the viral vector, it is possible to transfect insect cell lines or complete insect organisms.

The Baculovirus system is one of the most preferred and verstaile system for expresssion of recombinant proteins and it offers the following advantages.

1. Safety: Baculoviruses infect specific host insects and are essentially non infectious and are considered very safe to humans and plants.
2. No helper cell lines or viruses needed: The baculovirus genome contains all the complete genetic information necessary for propagation in insect cell lines.
3. Ease of Scale Up: Since the baculovirus is propagated using insect cell lines, many of these systems are already tested and amenable to suspension culture and scale-up using bioreactors.
4. High levels of soluble protein expression: In many cases the recombinant proteins that are expressed in the baculovirus system are found soluble. Obtaining soluble protein expression is ideal for many downstream applications such as crystallography and for those where the activity of the protein needs to be maintained.
5. Accuracy: Many of the post-translational modifications are correctly done in the baculovirus system.


Now you can have your own custom designed recombinant protein expressed  in Baculovirus system  and purified at a reasonable price! . Using our standardized transfection protocol we usually achieve ~ 99%  recombination efficiency. We offer to screen the plaques and can scale up and purify the protein from the selected plaque (s). We offer the complete package where we start with the gene and hand you the final purified protein from 1 liter culture or you may choose any of the services listed below.

Packages Description Price in US $
Package A    Subcloning your gene into a standard baculovirus transfer vector, sequence verification, co-transfecting with baculovirus DNA,  plaque screening, determine virus titer by limited dilution,  amplifying resulting virus and expression of protein in 1 liter culture, purification of the protein using the selected tag and analyzing protein by SDS-PAGE and western blot.   6990.00
   Generation of the recombinat baculovirus from PVL1392/1393  690.00
   Baculovirus plaque purification , amplify frpm 6 plaques and send to customer for evaluation  
   Baculovirus expansion and titer determination  690.00
  Baculovirus protein expression in 1 liter 500.00
   Baculovirus protein expression in 5 liters 2500.00
   Baculovirus protein expression in  14 liters 3995.00
  Baculovirus protein expression  in 36 liters  7800.00
  Affinity purification of expressed protein using Ni-NTA column - 1liter culture  690.00
  Affinity purification of expressed protein using Ni-NTA column - 5 liter culture  1490.00
  Pilot expression of your protein in insect larva This email address is being protected from spambots. You need JavaScript enabled to view it.
  large scale expression of your protein in insect larva This email address is being protected from spambots. You need JavaScript enabled to view it.



       

 

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