Denovo Biotechnology's 2x Hot Start PCR Master Mix is a ready-to-use solution for PCR and quantitative PCR (qPCR). It contains Taq DNA polymerase, dNTPs, and Taq buffer. It also contains an aptamer that inhibits Taq polymerase activity at low temperatures, so that the reaction can be set up at room temperature. This mixture is validated for qPCR and for PCR templates up to 5 kb.
This kit is recommended for use with Denovo Biotechnology's 20x probe-primer mixes for real time PCR.