- Description
- Specifications
Catalog #: ANT-180
Type:
Polyclonal Rabbit Antibody.
Applications:
This polyclonal antibody can be used for detection of SARS Nucleocapsid protein in western blot analysis at a dilution of 1:100-1:2000. It can also be used in cell staining.
Isotype:
Rabbit Ig.
Storage:
Store at 4 °C, stable for 6 months. For long-term storage, store at -200 °C.
Western blot analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using IMG-548 at a 1:2000 dilution.
Antigen:
The antibody was developed by immunizing rabbits with synthetic peptides corresponding to amino a.a. 399-411 of putative SARS nucleocapsid (Genbank accession no. NP_828858).
Western Blot:
Western blot analysis of SARS Nucleocapsid in (A) untransfected mouse melanoma cell lysate and (B) transfected cell lysate using IMG-548 at a 1:2000 dilution.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background:
It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.
Contents:
100µgs in 200µl PBS containing 0.2% gelatin and 0.05% sodium azide.
