- Description
- Specifications
Catalog #: HCV-264
Description:
The E.coli derived 19 kDa recombinant protein contains the HCV NS4 immunodominant regions, amino acids 1658-1863. The protein is fused with b-galactosidase (114 kDa) at N-terminus, pI 5.45.
Amino Acid Sequence:
1658 TWVLVGGVLAALAAYCLSTGCVVIVGRVVLSGKPAIIPDREVLYREF
DEMEECSQHLPYIEQGMMLAEQFKQKALGLLQTASRQAEVIAPAVQTNW
QKLETFWAKHMWNFISGIQYLAGLSTLPGNPAIASLMAFTAAVTSPLTTSQ
TLLFNILGGWVAAQLAAPGAATAFVGAGLAGAAIGSVGLGKVLIDILAGYGA
GVAGAL 1863.
Applications:
HCV NS4 a+b antigen is suitable for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.
Purification Method:
HCV NS4 a+b protein was purified by proprietary chromatographic technique.
Purity:
HCV NS4 a+b protein is >95% pure as determined by 10% PAGE (coomassie staining).
Formulation:
20mM Tris-Hcl pH 8, 8M urea.
Specificity:
Immunoreactive with sera of HCV-infected individuals.
Storage:
HCV NS4 a+b although stable at 4 °C for 1 week, should be stored below -18 °C.
Please prevent freeze thaw cycles.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
