- Description
- Specifications
Catalog #: HIV-146
Description:
HIV-1 gp120 LAV isolate Recombinant- is the external envelope protein, full-length 100-120 kDa, derived from the env. gene of HIV-1 and glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells. Purified under conditions that maintain the tertiary structure of the biologically active molecule. HIV-1 gp120 LAV sequence is identical to the predicted amino acid sequence of gp120 from pNL4-3 (Adachi et al. [1986], J. Virol. 59, 284-291; GenBank accession number M19921).
Source:
Baculovirus Insect Cells.
Amino Acid Sequence:
IPGEKLWVTV YYGVPVWKEA TTTLFCASDA KAYDTEVHNV ATHACVPTDP NPQEVVLVNV TENFNMWKND MVEQMHEDII SLWDQSLKPC VKLTPLCVSL KCTDLKNDTN TNSSSGRMIM EKGEIKNCSF NISTSIRDKV QKEYAFFYKL DIVPIDNTSY RLISCNTSVI TQACPKVSFE PIPIHYCAPA GFAILKCNNK TFNGTGPCTN VSTVQCTHGI RPVVSTQLLL NGSLAEEDVV IRSANFTDNA KTIIVQLNTS VEINCTRPNN NTRKSIRIQR GPGRAFVTIG KIGNMRQAHC NISRAKWNAT LKQIASKLRE QFGNNKTIIF KQSSGGDPEI VTHSFNCGGE FFYCNSTQLF NSTWFNSTWS TEGSNNTEGS DTITLPCRIK QFINMWQEVG KAMYAPPISG QIRCSSNITG LLLTRDGGNN NNGSEIFRPG GGDMRDNWRS ELYKYKVVKI EPLGVAPTKA KRRVVQREKR.
Applications:
HIV-1 gp120 antigen is suitable for ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
Purity:
Greater than 90.0% as determined by HPLC analysis and SDS-PAGE.
Formulation:
The protein solution contains 10mM Tris-Cl pH-7.6, 150mM NaCl and 0.01% Triton N-101.
Stability:
Recombinant HIV -1 gp120 LAV although stable at 4 °C for 3 weeks, should be stored desiccated below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Specificity:
Immunoreactive with sera from HIV infected individuals. Western blots: 0.1-1.0 µg/strip.
Physical Appearance:
Sterile filtered colorless clear solution.
Usage:
Denovo Biotechnology''s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
