- Description
- Specifications
Catalog #: CYT-500
Description:
Interleukin-10 Human Recombinant produced in E.coli is a single non-glycosylated polypeptide chains containing 161 amino acids each and having a molecular mass of 18.6kDa.
The IL-10 is purified by proprietary chromatographic techniques.
Synonyms:
B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
Source:
Escherichia Coli.
Amino Acid Sequence:
MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N.
Purity:
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Interleukin-10 in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized from a 0.2 µm filtered concentrated (1mg/ml) solution in PBS, pH 7.4.
Stability:
Lyophilized Interleukin-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL10 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50 as determined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells was found to be less than 2.0ng/ml, corresponding to a specific activity of 5.0x105 IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
1.Title:Activated signal transducer and activator of transcription-3 (STAT3) is a poor regulator of tumour necrosis factor- production by human monocytes.
Publication:Article first published online: 9 JAN 2007 DOI:10.1111/j.1365-2249.2006.03291.x
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2006.03291.x/full
2.Title:Suppressor of cytokine signalling-3 at pathological levels does not regulate lipopolysaccharide or interleukin-10 control of tumour necrosis factor-? production by human monocytes.
Publication:Article first published online: 11 MAY 2006 DOI: 10.1111/j.1365-2567.2006.02383.x
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2567.2006.02383.x/full
3.Title:The Tyrosine Sulfate-rich Domains of the LRR Proteins Fibromodulin and Osteoadherin Bind Motifs of Basic Clusters in a Variety of Heparin-binding Proteins, Including Bioactive Factors*.
Publication:First Published on August 21, 2009, doi: 10.1074/jbc.M109.047076 October 16, 2009 The Journal of Biological Chemistry, 284, 28543-28553.
Link:http://www.jbc.org/content/284/42/28543.full
