- Description
- Specifications
Catalog #: CYT-100
Description:
Interleukin-10 Human Recombinant produced in HEK cells is a glycosylated non-disulfide bonded homodimer, having a total molecular weight of 17kDa.
The IL10 is purified by proprietary chromatographic techniques.
Synonyms:
B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
Source:
HEK.
Purity:
Greater than 95% as obsereved by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized IL-10 in sterile water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The IL10 was lyophilized from 1mg/ml in 10mM Tris-HCl, pH 7.4 and 150mM NaCl.
Stability:
Lyophilized IL-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL10 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The specific activity was determined by the dose-dependent stimulation of the proliferation of MC/9 cells (mouse mast cell line) in the presence of 200 pg/ml IL-4, and is typically 0.3-1.5ng/ml.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
