- Description
- Specifications
Catalog #: CYT-230
Description:
Interleukin-15 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12773.5 Dalton.
The IL-15 is purified by proprietary chromatographic techniques.
Synonyms:
IL-15, MGC9721.
Source:
Escherichia Coli.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asn-Trp-Val-Asn.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Interleukin-15 in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The protein was lyophilized from 20mM NaHCO3 pH 8.5.
Stability:
Lyophilized Interleukin-15 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL15 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50 as determined by the dose-dependant stimulation of the proliferation of mouse CTLL-2 was found to be < 0.5 ng/ml, corresponding to a Specific Activity of 2 x 106 IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-15 as a Reference Standard.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
