- Description
- Specifications
Catalog #: CYT-655
Description:
Interleukin33 Mouse recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17.5 kDa.
Synonyms:
Interleukin 33, DVS27, NF-HEV, NKHEV, C9orf26, Interleukin-1 family member 11, IL- 1F11, Nuclear factor from high endothelial venules, Il-33, Il1f11, 9230117N10Rik, Il33.
Source:
Escherichia Coli.
Amino Acid Sequence:
SIQGTSLLTQ SPASLSTYND QSVSFVLENG CYVINVDDSG KDQEQDQVLL RYYESPCPAS QSGDGVDGKK LMVNMSPIKD TDIWLHANDK DYSVELQRGD VSPPEQAFFV LHKKSSDFVS FECKNLPGTY IGVKDNQLAL VEEKDESCNN IMFKLSKI.
Purity:
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized IL-33 in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The Mouse IL-33 was lyophilized from a concentrated (1mg/ml) solution containing 20mM Phosphate buffer pH-7.4, 150mM sodium chloride, 1mM EDTA and 2mM b-ME.
Stability:
Lyophilized IL-33 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL-33 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
Please prevent freeze-thaw cycles.
Activity:
The ED50 as determined by the dose-dependent stimulation of the proliferation of murine D10S cells is < 0.5ng/ml, corresponding to a specific activity of 2,000,000 units/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
