- Description
- Specifications
Catalog #: CYT-274
Description:
Interleukin-6 Human Recombinant produced in CHO is a single, glycosylated polypeptide chain containing 185 amino acids and migrates at 22 kDa as a glycosilated protein on SDS-PAGE.
The IL6 is purified by proprietary chromatographic techniques.
Synonyms:
IFN-b2, B cell differentiation factor, BCDF, BSF-2, HPGF, HSF, MGI-2, B-cell stimulatory factor 2, Interferon beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, IL-6, HGF.
Source:
Chinese Hamster Ovarian Cells.
Amino Acid Sequence:
APVPPGEDSK DVAAPHRQPL TSSERIDKQI RYILDGISAL RKETCNKSNM CESSKEALAE NNLNLPKMAE KDGCFQSGFN EETCLVKIIT GLLEFEVYLE YLQNRFESSE EQARAVQMST KVLIQFLQKK AKNLDAITTP DPTTNASLLT KLQAQNQWLQ DMTTHLILRS FKEFLQSSLR ALRQM.
Purity:
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Interleukin-6 in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized from a solution containing Phosphate- Buffered Saline pH-7.4.
Stability:
Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL6 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The activity was measured in a cell prolifiration assay using B9 cells. The ED50 for this effect is 0.008-0.02ng/ml corresponding to a Specific Activity of 50-125,000,000IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
