- Description
- Specifications
Catalog #: CYT-163
Description:
IL 7 Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 129 amino acids and having a molecular mass of 15.0kDa.
The IL 7 is purified by proprietary chromatographic techniques.
Synonyms:
Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7.
Source:
Escherichia Coli.
Amino Acid Sequence:
DCHIKDKDGK AFGSVLMISI NQLDKMTGTD SDCPNNEPNF FKKHLCDDTK EAAFLNRAAR KLRQFLKMNI SEEFNDHLLR VSDGTQTLVN CTSKEEKTIK EQKKNDPCFL KRLLREIKTC WNKILKGSI
Purity:
Greater than 98.0% as determined by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized IL-7 in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Stability:
Lyophilized IL-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IL-7 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
Please prevent freeze-thaw cycles.
Activity:
Fully biologically active when compared to standard. The ED50 was determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is less than 0.2ng/ml, corresponding to a specific activity of ?5,000,000IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.