- Description
- Specifications
Catalog #: PKA-219
Description:
Protein Tyrosine Phosphatase Non Receptor Type-1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 321 amino acids and having a molecular mass of 37.3 kDa.
Synonyms:
Tyrosine-protein phosphatase non-receptor type 1, EC 3.1.3.48, Protein-tyrosine phosphatase 1B, PTP-1B, PTPN1, PTP1B.
Source:
Escherichia Coli.
Amino Acid Sequence:
memekefeqi dksgswaaiy qdirheasdf pcrvaklpkn knrnryrdvs pfdhsriklhqedndyinas likmeeaqrs yiltqgplpn tcghfwemvw eqksrgvvml nrvmekgslkcaqywpqkee kemifedtnl kltlisedik syytvrqlel enlttqetre ilhfhyttwpdfgvpespas flnflfkvre sgslspehgp vvvhcsagig rsgtfcladt clllmdkrkdpssvdikkvl lemrkfrmgl iqtadqlrfs ylaviegakf imgdssvqdq wkelshedle pppehipppprppkrilephn.
Purity:
Greater than 95.0% as determined by SDS-PAGE.
Formulation:
The protein contains 25mM Tris-HCl, pH 7.5, 2mM b-mercaptoethanol, 1mM EDTA, 1mM DTT and 20% Glycerol.
Stability:
Store at 4 °C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 °C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Activity:
Specific activity: 6,000-12,000units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.
Physical Appearance:
Sterile filtered colorless solution.
Unit Definition:
One unit will hydrolyze 1 nanomole of p-nitrophenylphosphatate per minute at pH 7.4 at 37 °C using 10mM of substrate.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
