- Description
- Specifications
Catalog #: PKA-221
Description:
PTPN6 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 300 amino acids and having a molecular mass of 34.3 kDa. The protein coding region of the catalytic domain of PTPN6 (amino acids 243-541). The catalytic domain of PTPN6 was overexpressed as insoluble protein aggregates (inclusion bodies). The recombinant PTPN6 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid(Met) is attached at N-terminus.
Synonyms:
Tyrosine-protein phosphatase non-receptor type 6, EC 3.1.3.48, Protein-tyrosine phosphatase 1C, PTP-1C, Hematopoietic cell protein-tyrosine phosphatase, SH-PTP1, Protein-tyrosine phosphatase SHP-1, PTPN6, HCP, HCPH, SHP1, HPTP1C, SHP-1L.
Source:
Escherichia Coli.
Amino Acid Sequence:
Mgfweefes lqkqevknlh qrlegqrpen kgknryknil pfdhsrvilq grdsnipgsdyinanyiknq llgpdenakt yiasqgclea tvndfwqmaw qensrvivmt trevekgrnkcvpywpevgm qraygpysvt ncgehdttey klrtlqvspl dngdlireiw hyqylswpdhgvpsepggvl sfldqinqrq eslphagpii vhcsagigrt gtiividmlm enistkgldcdidiqktiqm vraqrsgmvq teaqykfiyv aiaqfiettk kklevlqsqk gqeseygnity.
Purity:
Greater than 95.0% as determined by(a)Analysis by RP-HPLC.
(b)Analysis by SDS-PAGE.
Formulation:
The protein contains 25mM Tris-HCl, pH 7.5, 2mM b-mercaptoethanol, 1mM EDTA, 1mMDTT and 20%Glycerol.
Stability:
Store at 4 °C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 °C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Activity:
5,000 U/mg.
Physical Appearance:
Sterile filtered colorless solution.
Unit Definition:
One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate per minute at pH 7.5 at 37 °C using 10mM of substrate.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
