- Description
- Specifications
Catalog #: PKA-366
Description:
ACVR1B Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain having a molecular weight of 38.2 kDa though on SDS-PAGE migrates at about 46 kDa due to the glycosilation. The ACVR1B sequence
(24-126 a.a) corresponds to the extracellular region of mature Human protein, fused to the Fc region of Human IgG1.
The ACVR1B is purified by proprietary chromatographic techniques.
Synonyms:
EC 2.7.11.30, ALK4, SKR2, ACVRLK4, ActRIB, Activin receptor type-1B .
Source:
HEK293 Cells.
Amino Acid Sequence:
SGPRGVQALL CACTSCLQAN YTCETDGACM VSIFNLDGME HHVRTCIPKV ELVPAGKPFY CLSSEDLRNT HCCYTDYCNR IDLRVPSGHL KEPEHPSMWG PVEENLYFQG GSGTKLDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHGDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK.
Purity:
Greater than 95.0% as determined by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized ACVR1B in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The ACVR1B protein was lyophilized from a 0.2 µm filtered solution containing no additives or preservatives.
Stability:
Lyophilized ACVR1B although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution ACVR1B should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
In a functional ELISA, immobilized recombinant Human ACVR1B (0.2µg/mL) will bind recombinant Human ACVR1B with a linear range of 0.8 – 100ng/mL.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
