- Description
- Specifications
Catalog #: PKA-309
Description:
ELK-1 (amino acids 307-428) Human Recombinant is produced in E.Coli as a maltose binding protein (MBP) fusion protein with an amino-terminal polyhistidine tag and a c-terminal myc tag (EQKLISEEDL), and having a molecular mass of 60 kDa.
Elk-1 is purified by proprietary chromatographic techniques.
Synonyms:
ETS domain-containing protein Elk-1, ELK1, Elk-1.
Source:
Escherichia Coli.
Purity:
Greater than 95% as determined by SDS-PAGE.
Formulation:
ELK-1 is supplied as lyophilized freeze dry powder without additives.
Activity:
Recombinant human Elk-1 is phosphorylatable in vitro, using either recombinant active ERK1/2, or with immunoprecipitated ERK1/2 from stimulated cells. This phosphorylation can be monitored by Western blot analysis. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.
Note:
Kinase activity may vary depending on the substrate and reaction conditions.
Storage:
Store at 2-8 °C if entire vial will be used within 1-2 weeks. Store, frozen at -20 °C for longer periods of time. Avoid multiple freeze-thaw cycles.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
