- Description
- Specifications
Catalog #: PKA-305
Description:
AKT1 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain containing 481 amino acids and having a molecular mass of 60 KD. This protein is the full-length form of the protein with a N-terminal His-tag.
AKT1 is purified by proprietary chromatographic techniques.
Synonyms:
RAC-alpha serine/threonine-protein kinase, EC 2.7.11.1, RAC-PK-alpha, Protein kinase B, C-AKT, AKT, PKB, RAC, PRKBA, MGC99656, RAC-ALPHA.
Source:
Baculovirus, SF9 insect cells.
Purity:
Greater than 95% as determined by SDS-PAGE.
Mass spectrometry of tryptic digest products for identity determination.
Formulation:
AKT1 (0.1mg/ml) is supplied in 50mM Tris pH 7.5, 0.15M NaCl, 0.27M sucrose,10mM beta-mercaptoethanol, 1mM EGTA. Carrier-free.
Physical Appearance:
Sterile Filtered clear solution.
Storage:
Store at 4 °C if entire vial will be used within 1-2 weeks. Store, frozen at -20 °C- -80 °C for longer periods of time. Avoid multiple freeze-thaw cycles.
Unit Definition:
250 units/mg. One unit of Akt1 activity is equal to 1 nanomole of phosphate transferred to crosstide substrate per minute at 30 °C with a final ATP concentration of 100 µM. The optimal concentration should be determined for each specific application.
Please note: Kinase activity may vary depending on the substrate and reaction conditions.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
