- Description
- Specifications
Catalog #: CYT-421
Description:
Stem Cell Factor Human Recombinant produced in insect cells is a single, glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton.
The SCF is fused to a C-terminal His-tag (6x His) and purified by proprietary chromatographic techniques.
Synonyms:
Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.
Source:
Sf9, Insect Cells.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Stem Cell Factor in 10 mM acetic acid not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The protein is supplied in 1X PBS, pH 7.4.
Stability:
Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution SCF should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is typically 2-5ng/ml, corresponding to a Specific Activity of 200,000-500,000units/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
