- Description
- Specifications
Catalog #: ENZ-380
Description:
LCAT Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 426 amino acids (25-440) which includes a 10 amino acid His Tag fused at N-terminus and having a total molecular mass of 48.3 kDa.
LCAT Human Recombinant is purified by proprietary chromatographic techniques.
Synonyms:
Phosphatidylcholine-sterol acyltransferase, Lecithin-cholesterol acyltransferase, Phospholipid-cholesterol acyltransferase, LCAT.
Source:
Escherichia Coli.
Amino Acid Sequence:
MKHHHHHHAS FWLLNVLFPP HTTPKAELSN HTRPVILVPG CLGNQLEAKL DKPDVVNWMC YRKTEDFFTI WLDLNMFLPL GVDCWIDNTR VVYNRSSGLV SNAPGVQIRV PGFGKTYSVE YLDSSKLAGY LHTLVQNLVN NGYVRDETVR AAPYDWRLEP GQQEEYYRKL AGLVEEMHAA YGKPVFLIGH SLGCLHLLYF LLRQPQAWKD RFIDGFISLG APWGGSIKPM LVLASGDNQG IPIMSSIKLK EEQRITTTSP WMFPSRMAWP EDHVFISTPS FNYTGRDFQR FFADLHFEEG WYMWLQSRDL LAGLPAPGVE VYCLYGVGLP TPRTYIYDHG FPYTDPVGVL YEDGDDTVAT RSTELCGLWQ GRQPQPVHLL PLHGIQHLNM VFSNLTLEHI NAILLGAYRQ GPPASPTASPEPPPPE.
Purity:
Greater than 95.0% as determined by SDS-PAGE.
Solubility:
Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Formulation:
The LCAT protein was lyophilized from 0.4µm filtered solution at a concentration of 0.5mg/ml containing 0.05M Acetate Buffer pH-4.0.
Stability:
Lyophilized LCAT although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution LCAT should be stored at 4 °C between 2-7 days and for future use below
-18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Physical Appearance:
Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
