Custom Donkey Antibody
One donkey is immunized via intramuscular injection with client supplied antigen. Towards the end of the protocol, 3 production bleeds are taken and the animals are placed on rest pending your testing results.
Each production bleed will average approximately 350 ml serum per animal and exsanguinations yield approximately 2000 ml serum per animal. In either case, you can expect approximately 20 - 30 mg/ml serum antibody. The antibody can be purified via several common methods, but DEAE sepharose is the most common.
We will need 2 milligram of your antigen to complete the 1 animal protocol. Immunoaffinity purification or protocol extensions may require additional antigen.
| Day | Procedure |
| 1 | 1 ml serum sample pre-immunization. Inject 0.5 ml antigen mixed with 0.5 ml Complete Freund's adjuvant. |
| 21 | Inject 0.5 ml antigen mixed with 0.5 ml Incomplete Freund's adjuvant. |
| 42 | Inject 0.5 ml antigen mixed with 0.5 ml Incomplete Freund's adjuvant. |
| 54 | Test bleed. Sample shipped for titer testing/screening (or we can test it here). |
| 61 | Inject 0.5 ml antigen mixed with 0.5 ml Incomplete Freund's adjuvant. |
| 70 | Production bleed. |
| 75 | Inject 0.5 ml antigen mixed with 0.5 ml Incomplete Freund's adjuvant. |
| 85 | Production bleed. |
| 103 | Production bleed. |
| Monthly | Boost and bleed indefinitely |
When the project is completed and serum is collected, the project will automatically go on maintenance at $270.00 per animal per month unless you instruct us otherwise.
Need more information? This email address is being protected from spambots. You need JavaScript enabled to view it.
