- Description
- Specifications
Catalog #: PKA-306
Description:
Activating Transcription Factor-2 Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain encoding 19-96 amino acids and having a molecular mass of 57 kDa.
The ATF-2 is manufactured as a maltose binding protein (MBP) fusion protein with an amino terminal polyhistidine tag.
ATF-2 is purified by proprietary chromatographic techniques.
Synonyms:
Cyclic AMP-dependent transcription factor ATF-2, Activating transcription factor 2, cAMP response element-binding protein CRE-BP1, HB16, CREB2, TREB7, MGC111558, ATF2.
Source:
Escherichia Coli.
Purity:
Greater than 90% as determined by SDS-PAGE.
Formulation:
ATF-2 is supplied as lyophilized freeze dry powder without additives.
Storage:
Store at 4 °C if entire vial will be used within 1-2 weeks. Store, frozen at -20 °C for longer periods of time. Avoid multiple freeze-thaw cycles.
Unit Definition:
ATF2 is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with immunoprecipitated JNK from stimulated cells. This phosphorylation can be monitored by Western blot analysis using phosphorylation site specific antibody directed to ATF2 [pT71], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.
Please note: Kinase activity may vary depending on the substrate and reaction conditions.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
