- Description
- Specifications
Catalog #: CYT-057
Description:
Activin B human Recombinant produced in Nicotiana benthamiana plant is a ?B single chain containing 123 amino acids (molecular formula C615H910N178O177S12).
Activin B is fused to a 10-His-tag at the N-terminal having the total molecular mass of 14kDa and purified by standard chromatographic techniques.
Synonyms:
Inhibin beta B (activin AB beta polypeptide), Inhibin, beta-2, Activin beta-B chain, MGC157939.
Source:
Nicotiana benthamiana plant
Amino Acid Sequence:
HHHHHHHHHH GLECDGRTNL CCRQQFFIDF RLIGWNDWII APTGYYGNYC EGSCPAYLAG VPGSASSFHT AVVNQYRMRG LNPGTVNSCC IPTKLSTMSM LYFDDEYNIV KRDVPNMIVE ECG
Purity:
Greater than 97.0% as determined by Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Activin B in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized from 1mg/ml solution in Tris HCl 0.05M buffer at pH 7.4.
Stability:
Lyophilized Activin B although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution Activin B should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The biological activity of Activin B is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation.
<5ng/mL are required to stimulate a half-maximal response at cytokine saturation. Note: Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Serological Identification:
The protein was electrophoresed under reducing condition on a 15% SDS-polyacrylamide gel, transferred by electro blotting to a NC membrane and visualized by immune-detection with specific Activin B antibody.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
