- Description
- Specifications
Catalog #: CYT-233
Description:
Trimeric form of Adiponectin Human trimeric form was expressed in HEK293 cells. The cysteine 39 was replaced with Alanine (C39A) 9. hAd-C39A can only form a trimer, but not a hexamer or an HMW form.
Synonyms:
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Source:
HEK293 (Human embryonic kidney cell line).
Amino Acid Sequence:
ETTTQGPGVL LPLPKGAATG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDD DDK.
Applications:
In vitro, ex vivo and in vivo activity analysis, binding assay et al.
Purity:
Greater than 95% as determined by SDS-PAGE.
Solubility:
Add deionized water to a working volume of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Formulation:
Filtered (0.4 µm) and lyophilized from 0.5 mg/ml in 0.05M phosphate buffer, 0.05M NaCl, pH 7.2.
Stability:
Store lyophilized Adiponectin at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 °C for a limited period of time; it does not show any change after two weeks at 4 °C.
Activity:
ED50= 3-8.5 µg/ml, as determined by its ability to inhibit proliferation of HASMCs induced by HB EGF.
Physical Appearance:
Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Patent Rights:
The sale and/or commercial use of Recombinant Adiponectin is prohibited in the United States of America (U.S.A).
