- Description
- Specifications
Catalog #: CYT-435
Description:
The Acrp30 Mouse Recombinant is fused with FLAG tag having a total Mw of 26kDa.
Synonyms:
Acrp30, AdipoQ, GBP-28, APM-1, ACDC.
Source:
HEK293 (Human embryonic kidney cell line).
Amino Acid Sequence:
EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYMYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN DYKDDDDK.
Applications:
ELISA
Western blotting
Cell culture and/or animal studies.
Purity:
Purity of adiponectin mouse recombinant is >98% (HPLC and SDS PAGE analyzed).
Solubility:
Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.
Formulation:
Sterile filtered and lyophilized from 0.5 mg/ml in PBS buffer.
Stability:
Store lyophilized APM-1 at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 °C for a limited period of time; it does not show any change after two weeks at 4 °C.
Activity:
Full-length mouse adiponectin activates AMP-activated protein kinase in hepatocyte and activates AMPK in HepG2 human hepatocytes at a concentration of 1000ng/ml corresponding to a Specific Activity of 1000IU/mg. Mouse adiponectin mammalian cell derived inhibits glucose production as shown by in-vitro gluconeogenesis assay in primary rat hepatocytes.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Patent Rights:
The sale and/or commercial use of Recombinant Adiponectin is prohibited in the United States of America (U.S.A).
