- Description
- Specifications
Catalog #: CYT-278
Description:
The Clusterin Human contains a total 438 amino acids and a calculated molecular mass of 51.27kDa (calculated). The AA sequence (AA 1-427) is identical to Swiss-Prot-P10909 (AA 23-449, secreted Human Clusterin). C-terminal Flag-tag 11 extra AA (underlined).
Synonyms:
CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Clusterin, Apolipoprotein J, Apo-J.
Source:
293 cell line (Human embryonic kidney).
Amino Acid Sequence:
DQTVSDNELQ EMSNQGSKYV NKEIQNAVNG VKQIKTLIEK TNEERKTLLS NLEEAKKKKE DALNETRESE TKLKELPGVC NETMMALWEE CKPCLKQTCM KFYARVCRSGS GLVGRQLEE FLNQSSPFYF WMNGDRIDSL LENDRQQTHM LDVMQDHFSRA SSIIDELFQ DRFFTREPQD TYHYLPFSLP HRRPHFFFPK SRIVRSLMPF SPYEPLNFHA MFQPFLEMIH EAQQAMDIHF HSPAFQHPPT EFIREGDDDR TVCREIRHNS TGCLRMKDQC DKCREILSVD CSTNNPSQAKLRRELDESLQ VAERLTRKYN ELLKSYQWKM LNTSSLLEQL NEQFNWVSRL ANLTQGEDQYYLRVTTVASH TSDSDVPSGV TEVVVKLFDS DPITVTVPVE VSRKNPKFME TVAEKALQEY RKKHREEAAA DYKDDDDK.
Purity:
Greater than 95% as determined by SDS PAGE.
Solubility:
Add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product not sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.
Formulation:
Filtered (0.4 micron) and lyophilized PBS, pH 7.5.
Stability:
Store lyophilized protein at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 °C for a limited period of time; it does not show any change after two weeks at 4 °C.
Physical Appearance:
Filtered, White, Lyophilized powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
Title:Clusterin Facilitates Exchange of Glycosyl Phosphatidylinositol-Linked SPAM1 Between Reproductive Luminal Fluids and Mouse and Human Sperm Membranes1.
Publication:BIOLOGY OF REPRODUCTION 81, 562–570 (2009) Published online before print 8 April 2009. DOI 10.1095/biolreprod.108.075739
Link:http://www.biolreprod.org/content/81/3/562.full.pdf
