- Description
- Specifications
Catalog #: PRO-630
Description:
Bcl-2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing amino acids 1-206.
The wild type Bcl-2 is missing 12 amino acids from C-terminus. C-terminus is fused to His-Tag. C-terminus his-tag, mimics the deleted C-terminus membrane domain thus maintaining its biological activity.
Bcl-2 is purified by proprietary chromatographic techniques.
Synonyms:
Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.
Source:
Escherichia Coli.
Applications:
Input marker or positive control (Western Blotting).
Function study.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
Suspend Bcl-2 in 100ul of 0.5M Acetic acid, over night at 4 °C.
Dilute 10 fold into selected buffer system.
BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.
Formulation:
The protein contains 10mM Tris-HCl pH-8, 1mM EDTA and 250mM NaCl.
Stability:
Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution Bcl-2 should be stored at 4 °C between 2-7 days and for future use below
-18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
