- Description
- Specifications
Catalog #: CYT-062
Description:
BD-1 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 37 amino acids and having a molecular mass of 4.1kDa.
The BD-1 is purified by proprietary chromatographic techniques.
Synonyms:
Beta-defensin 1, BD-1, rBD-1, Defensin beta 1, Defb1.
Source:
Escherichia Coli.
Amino Acid Sequence:
DQYRCLQNGG FCLRSSCPSH TKLQGTCKPD KPNCCRS.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized BD-1 in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
BD-1 protein was lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Stability:
Lyophilized BD-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution BD-1 should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
Measured by its ability to chemoattract CD34+ dendritic cells using a concentration range of 0.1-1.0 ug/ml.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.