- Description
- Specifications
Catalog #: CYT-079
Description:
Beta-NGF Human Recombinant produced in HEK cells is a non-glycosylated non-disulfide linked homodimer, having a total molecular weight of 13kDa.
The b-NGF is purified by proprietary chromatographic techniques.
Synonyms:
Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.
Source:
HEK.
Purity:
Greater than 95% as obsereved by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized b-NGF in sterile water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The b-NGF was lyophilized from 1mg/ml in 1xPBS.
Stability:
Lyophilized b-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution b-NGF should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) and is typically 1.5-8ng/ml.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
