- Description
- Specifications
Catalog #: CYT-440
Description:
NGF beta Mouse produced in Submaxillary Gland of Grown Mouse is a homodimer, non-glycosylated, polypeptide chain containing 2 identical 120 amino acids and having a molecular mass of 13,471 Dalton each.
The NGF beta Mouse is purified by advanced biology purification technology.
Synonyms:
Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.
Source:
Submaxillary Gland of Grown Mouse.
Amino Acid Sequence:
SSTHPVFHMGEF SVCDSVSVWV GDKTTATDIK GKEVTVLAEV NINNSVFRQY FFETKCRASN PVESGCRGID SKHWNSYCTT THTFVKALTT DEKQAAWRFI RIDTACVCVL SRKATRRG.
Purity:
Greater than 98% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE .
Solubility:
It is recommended to reconstitute the lyophilized Murine NGF-beta in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The NGF beta Mouse was lyophilized from solution containing 5% mannitol and 1% HSA.
Stability:
Lyophilized Mouse Beta-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution Murine NGF-Beta should be stored at 4 °C between 2-7 days and for future use below -18 °C.
Please prevent freeze-thaw cycles.
Activity:
The method used to test the bioassay is the NGF-dependent survival of dorsal root ganglia neurons of chick embryo, corresponding to a Specific Activity of 500,000IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
V aron S, Raibo rn C. Dissociation, fractionation and culture ofchick embryo sympathetic ganglionic cells [ J ]. J N eu rocy tol, 1972; 1: 211- 221.
