- Description
- Specifications
Catalog #: CYT-361
Description:
Bone Morphogenetic Protein-4 Human Recombinant produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 116 amino acids and having a molecular mass of 13009 Dalton.
The BMP-4 is purified by proprietary chromatographic techniques.
Synonyms:
BMP4, ZYME, BMP2B, BMP2B1.
Source:
Escherichia Coli.
Amino Acid Sequence:
SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP
FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV
VLKNYQEMVV EGCGCR.
Applications:
1. Molecular standard (Western, ELISA) in studying secreted BMP-4.
2. Preparing antibodies for BMP-4 monomer.
3. Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.
Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-4 in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
BMP-4 was lyophilized from a 0.2 µm filtered concentrated (1mg/ml) solution in 30% Acetonitrile and 0.1% TFA.
Stability:
Lyophilized Bone Morphogenetic Protein-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution BMP4 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
ProsSec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
