- Description
- Specifications
Catalog #: CYT-276
Description:
N-TERMINAL---Human BMP-2 (Met 1 – Arg 282) Human BMP-7 (Ser 293 – Arg 431)---C-TERMINAL. The DNA sequence encoding the human BMP-2 signal peptide and propeptide (1~282 amino acid) fused to the human rhBMP-7 mature chain (293~431 amino acid) was expressed in a Chinese hamster ovary cell line. The mature recombinant BMP-7 generated by the proteolytic removal of the signal peptide and propetide contains 139 amino acid residues. The glycosylation of BMP-7 increases the molecular mass and the glycosylated proteins migrate as 25 ~ 40 kDa in SDS-PAGE under non-reducing conditions.
BMP-7 is purified by proprietary chromatographic techniques.
Synonyms:
Osteogenic Protein 1, BMP-7.
Source:
Chinese Hamster Ovarian Cells.
Purity:
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-7 in 1ml sterile/ endotoxin free water.
Formulation:
BMP-7 was lyophilized from a concentrated (1mg/ml) sterile solution containing 1% sucrose, 1.2% mannitol, 20mM glycine, and 0.05% tween 20 pH-4.
Stability:
Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution BMP 7 Human should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
Measured in alkaline phosphatase activity assay using MC3T3-E1 cells. The ED50 for this effect is <100ng/ml, corresponding to a Specific Activity of 10,000IU/mg.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
1.Title:Bone morphogenetic protein 7 induces mesenchymal-to-epithelial transition in melanoma cells, leading to inhibition of metastasis.
Publication:Article first published online: 30 JUL 2009 DOI:10.1111/j.1349-7006.2009.01301.x © 2009 Japanese Cancer Association.
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1349-7006.2009.01301.x/full
2.Title:In Vivo Degradation of a Novel Poly(?-Caprolactone) Invertebral Cage for Anterior Cervical Discectomy and Fusion in a Porcine Model.
Publication:Tissue Engineering and Regenerative Medicine International Society – EU Meeting -2010Galway, Ireland
Link:http://64.130.18.83/docs/eu10_abstracts/TERMIS2010_0708.pdf
3.Title:Pretreatment With Bone Morphogenetic Protein-7 (Bmp-7) Mimics Ischemia Preconditioning Following Intestinal Ischemia/Reperfusion Injury in the Intestine and Liver.
Publication:November 2008 - Volume 30 - Issue 5 - pp 532-536 doi: 10.1097/SHK.0b013e31816f20f1
Basic Science Aspects.
Link:http://journals.lww.com/shockjournal/Abstract/2008/
11000/Pretreatment_With_Boneµmorphogenetic_Protein_7.8.aspx
