- Description
- Specifications
Catalog #: PRO-390
Description:
CENPB Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 65,940 Dalton.
CENPB is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Synonyms:
Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB.
Source:
Sf9 insect cells.
Applications:
Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
Purity:
Greater than 90% as determined by SDS-PAGE.
Formulation:
CENPB is supplied in 20mM HEPES buffer pH-7.6, 250mM sodium chloride, and 20% glycerol.
Physical Appearance:
Sterile Filtered liquid formulation.
Coating Concentration:
0.15-0.375 µg/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Immunological Functions:
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Storage:
Store at 4 °C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 °C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
