- Description
- Specifications
Catalog #: CYT-654
Description:
CNTF Recombinant Rat produced in E.Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 22834 Dalton.
The CNTF is purified by proprietary chromatographic techniques.
Synonyms:
HCNTF, CNTF, Ciliary Neurotrophic Factor.
Source:
Escherichia Coli.
Amino Acid Sequence:
AFAEQTPLTL HRRDLSSRSIWLARKIRSDLTALMESYVKHQGLNKNI
NLDSVDGVPVASTDRWSEMTEAERLQENLQAYRTFQGMLTKLLEDQRV
HFTPTEGDFHQAIHTLMLQVSAFAYQLEELMVLLEQKIPENEADGMPA
TVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRVISSHQMGISALESH
YGAKDKQM.
Purity:
Greater than 99.0% as determined by:
(a) Analysis by Gel Filtration.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized CNTF in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100 µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Formulation:
Lyophilized from a concentrated (1mg/ml) solution in water containing 0.025% NaHCO3.
Stability:
Lyophilized CNTF although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution CNTF should be stored at 4 °C between 2-7 days and for future use below
-18 °C.
Please prevent freeze-thaw cycles.
Activity:
Fully biologically active by its ability to phosphorylate STAT3 in several cells lines.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein Content:
CNTF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.22 as the extinction
coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of CNTF Recombinant as a Reference Standard.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
