- Description
- Specifications
Catalog #: PRO-387
Description:
Human a3 chain of collagen IV; identical with the antigen called glomerular basal membrane antigen" (GBM). Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays. Binds IgG-type human auto-antibodies. Calculated Molecular weight: 43,591 Dalton. Calculated isoelectric point: pH 8.9. cDNA coding for a minicollagen version of the human collagen IV a3 chain fused to a hexa-histidine purification tag. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region (situated between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain), which is a requirement for recombinant production of this antigen.
Synonyms:
Collagen alpha-3(IV) chain, Goodpasture antigen, COL4A3, Glomerular Basal Membrane, GBM.
Source:
Sf9 insect cells.
Applications:
Standard ELISA test
Immuno-Dot test with positive/negative sera panels.
SDS-PAGE
Western-Blot.
Formulation:
The protein solution (0.6 mg/ml) contains 20mM Hepes, pH 8.0, and 4M Urea
Stability:
Recommendations for storage buffer: Ionic strength between 50 and 100 mM, neutral to slightly alkaline pH and 4 M urea as dissociating agent.
Storage temperature is -70 ° to -80 °C.
Please prevent freeze-thaw cycles.
Physical Appearance:
Sterile Filtered clear solution.
Coating Concentration:
0.12-0.5 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Caution:
It has been reported that the immunodominant epitope of COL4A3 is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes (see Hellmark et al. in Autoantibodies, Peter, J.B. and Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298).
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
