- Description
- Specifications
Catalog #: CYT-018
Description:
Granulocyte Colony Stimulating Factor Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18.8kDa. The Pegylated G-CSF is produced by attaching a 20kDa methoxypolyethylene glycol propionaldehyde (mPEG-ALD) to the N-terminal amino acid of G-CSF giving a total molecular mass of 38.8kDa.
G-CSF is purified by proprietary chromatographic techniques.
Synonyms:
CSF-3, MGI-1G, GM-CSF beta, Pluripoietin, Filgrastim, Lenograstim, G-CSF, MGC45931, GCSF.
Source:
Escherichia Coli.
Purity:
Greater than 95.0% as determined by SEC-HPLC.
Formulation:
G-CSF is supplied in solution (0.69mg/ml) containing 10mM Acetate Buffer (pH 4.0), and 0.004% Polysorbate 80.
Stability:
G-CSF PEG should be stored refrigerated at 2 ° to 8 °C. Vials should be kept in theirpackaging to protect from light until the time of use.
Shaking and freezing should be avoided.
Activity:
The ED50, calculated by the dose-dependent proliferation of murine NFS-60 indicator cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000IU/mg.
Physical Appearance:
Colorless, clear and transparent solution.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
