- Description
- Specifications
Catalog #: CYT-046
Description:
MCSF Rat Recombinant produced in HEK-293 cells is a secreted protein (amino acids Glu33-Arg254). M-CSF is disulfide-linked homodimer containing 2 x 222 a.a chains.
Synonyms:
CSF-1, Lanimostim, MCSF, MGC31930, M-CSF.
Source:
HEK293
Purity:
Greater than 95.0% as determined by SDS-PAGE.
Solubility:
It is recommended to reconstitute the MCSF in sterile PBS not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The recombinant MCSF was lyophilized after extensive dialysis against PBS.
Stability:
Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution MCSF should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50 of 3-4ng/ml is measured by its ability to stimulate the proliferation of mouse M-NFS-60 cells.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.