- Description
- Specifications
Catalog #: PRO-961
Description:
Cystatin-C Human Recombinant produced in HEK cells is a non-glycosylated monomer, having a molecular weight of approximately 13kDa.
The Cystatin-C is purified by proprietary chromatographic techniques.
Synonyms:
Cystatin-C, Cystatin-3, Neuroendocrine basic polypeptide, Gamma-trace, Post-gamma-globulin, CST3, MGC117328.
Source:
HEK.
Purity:
Greater than 95% as obsereved by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Cystatin-C in sterile water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The Cystatin-C was lyophilized from 1mg/ml in 1xPBS.
Stability:
Lyophilized Cystatin-C although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution Cystatin-C should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The inhibitory function of Cystatin-C on papin's protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5-20 µg/ml (0.3-1.5 µM) with a range of 1.56-50 µg/ml Cystatin-C in presence of 0.55 µM papain and 0.44 µM L-BAPA. The activity is typically 0.3-1.5 µM IC50.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
