- Description
- Specifications
Catalog #: ENZ-399
Description:
G6PD E.Coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 491 amino acids and having a molecular mass of 55.7kDa.
The G6PD is purified by proprietary chromatographic techniques.
Synonyms:
G6PD, G6PD1, Glucose-6-phosphate 1-dehydrogenase.
Source:
Escherichia Coli.
Amino Acid Sequence:
MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVDSITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E.
Purity:
Greater than 90.0% as determined by SDS-PAGE.
Formulation:
The G6PD protein contains 50mM MES 6.0, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT and 10% glycerol.
Stability:
Store at 4 °C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 °C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Activity:
Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
Physical Appearance:
Sterile filtered colorless solution.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
