- Description
- Specifications
Catalog #: CYT-669
Description:
Epidermal Growth Factor Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6151 Dalton.
The Rat EGF is purified by proprietary chromatographic techniques.
Synonyms:
Urogastrone, URG, EGF.
Source:
Escherichia Coli.
Amino Acid Sequence:
NSNTGCPPSY DGYCLNGGVC MYVESVDRYV CNCVIGYIGE RCQHRDLRWW KLR.
Purity:
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Rat EGF in sterile water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Rat EGF was lyophilized from a 0.2 µm filtered concentrated (1.0mg/ml) solution in PBS, pH 7.4.
Stability:
Lyophilized Rat EGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution Rat EGF should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50 as calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of > 10,000,000 units/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
