- Description
- Specifications
Catalog #: CYT-201
Description:
Erythropoietin-alpha Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 166 amino acids and having a predicted molecular mass of 21,000 Dalton and apparent glycosylated molecular mass of 36-40kDa Dalton. EPO-a is purified by proprietary chromatographic techniques.
Synonyms:
Erythropoietin-Alpha, EPO-a, EPO-alpha, Epoetin, EP, MGC138142.
Source:
Chinese Hamster Ovary Cells(CHO).
Amino Acid Sequence:
APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR.
Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Epoetin-a in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Each mg of lyophilized powder contains 0.59 mg sodium citrate, 0.58 mg sodium chloride and 0.006 mg citric acid.
Stability:
Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution EPO-alpha should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The Specific Activity was measured by Normocyth -aemic mice and was found to be 150,000 IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
1.Title:Zinc Transporters ZnT1 (Slc30a1), Zip8 (Slc39a8), and Zip10 (Slc39a10) in Mouse Red Blood Cells Are Differentially Regulated during Erythroid Development and by Dietary Zinc Deficiency1–3
Publication:Journal of Nutrition, doi:10.3945/jn.108.093575
Vol. 138, No. 11, 2076-2083, November 2008
© 2008 American Society for Nutrition J. Nutr. 138:2076-2083, November 2008
Link:http://jn.nutrition.org/content/138/11/2076.full
2.Title:Systemically delivered Erythropoietin transiently enhances adult hippocampal neurogenesis.
Publication:Article first published online: 7 MAY 2007 DOI:10.1111/j.1471-4159.2007.04684.x Journal of Neurochemistry Volume 102, Issue 6, pages 1953–1965, September 2007.
Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2007.04684.x/full
3.Title:ZINC TRANSPORTER EXPRESSION IN MATURE RED BLOOD CELLS AND
DIFFERENTIATING ERYTHROID PROGENITOR CELLS.
Publication:UNIVERSITY OF FLORIDA
© 2007 Moon-Suhn Ryu
Link:http://ufdcimages.uflib.ufl.edu/UF/E0/02/14/46/00001/ryuµm.pdf
4.Title:Cell Therapy with Human Renal Cell Cultures Containing Erythropoietin-Positive Cells Improves Chronic Kidney Injury.
Publication:First Published Online May 3, 2012 doi: 10.5966/sctm.2011-0048 Stem Cells Trans Med May 2012 vol. 1 no. 5 373-383
Link:http://stemcellstm.alphamedpress.org/content/1/5/373.full
