- Description
- Specifications
Catalog #: PRO-444
Description:
EXOSC10 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 120 kDa.
EXOSC10 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Synonyms:
Exosome component 10, Polymyositis/scleroderma autoantigen 2, Autoantigen PM/Scl 2, Polymyositis/scleroderma autoantigen 100 kDa, PM/Scl-100, P100 polymyositis-scleroderma overlap syndrome-associated autoantigen, EXOSC10, PMSCL, PMSCL2, p2, p3, p4, RRP6, Rrp6p, PM-Scl.
Source:
Sf9 insect cells.
Applications:
Western-Blot with monoclonal anti-hexa-His-tag antibody & polymyositis patient sera.
Purity:
Greater than 70% as determined by SDS-PAGE.
Formulation:
EXOSC10 is supplied at a concentration of 0.8mg/ml in 20mM HEPES, pH-7.5, 100mM NaCl, 0.01mM EDTA, 0.02% SDS.
Physical Appearance:
Sterile Filtered clear solution.
Coating Concentration:
0.4-0.8 µg/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Immunological Functions:
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels).
Storage:
Store at 4 °C if entire vial will be used within 2-4 weeks.
Store, frozen at -20 °C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
