- Description
- Specifications
Catalog #: CYT-176
Description:
FGF18 Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 21.0kDa.
The FGF 18 Rat is purified by proprietary chromatographic techniques.
Synonyms:
Fibroblast growth factor 18, FGF-18, zFGF5, Fgf18, D130055P09Rik.
Source:
Escherichia Coli.
Amino Acid Sequence:
EENVDFRIHV ENQTRARDDV SRKQLRLYQL YSRTSGKHIQ VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQTELQK PFKYTTVTKR SRRIRPTHPG
Purity:
Greater than 95.0% as determined by SDS-PAGE and HPLC analyses.
Solubility:
It is recommended to reconstitute the lyophilized FGF 18 in sterile 18M?-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.
Stability:
Lyophilized FGF 18 Rat although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution FGF 18 Rat should be stored at 4 °C between 2-7 days and for future use below -18 °C.
Please prevent freeze-thaw cycles.
Activity:
The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is ? 0.5ng/ml, corresponding to a specific activity of ? 2.0x1,000,000 units/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
