Fibroblast Growth Factor-Acidic Rat Recombinant - 50µg

Catalog #: CYT-075

Description:
Fibroblast Growth Factor-acidic Rat Recombinant (FGF-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15.9 kDa.
The FGF acidic is purified by proprietary chromatographic techniques.

Synonyms:
Fibroblast growth factor 1, FGF-1, Acidic fibroblast growth factor, aFGF, Heparin-binding growth factor 1, HBGF-1, Fgf1, Fgfa, HBGF1.

Source:
Escherichia Coli.

Amino Acid Sequence:
MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.

Purity:
Greater than 98.0% as determined by SDS-PAGE.

Solubility:
It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-acidic in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Formulation:
Lyophilized at a concentration of 1 mg/ml in 5mM Na₂PO₄, pH-7.5 and 50mM NaCl.

Stability:
Lyophilized Fibroblast Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution FGF-a should be stored at 4 °C between 2-7 days and for future use below -18 °C.
Please prevent freeze-thaw cycles.

Activity:
The ED50 as determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells, is less than 0.2ng/ml corresponding to a Specific Activity of 5x10⁶IU/mg.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.