Flt3 Ligand Human Recombinant - 10µg

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Catalog #: CYT-331Description:Flt3-Ligand Human Recombinant produced in E.Coli is non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17605 Dalton.Flt3-Ligand is purified by proprietary chromatographic techniques.Synonyms:Fms-related tyrosine kinase 3 ligand ...Read more
Catalog # CYT-331 $225.00 each


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  • Description
  • Specifications

Catalog #: CYT-331

Description:
Flt3-Ligand Human Recombinant produced in E.Coli is non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17605 Dalton.

Flt3-Ligand is purified by proprietary chromatographic techniques.

Synonyms:
Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.

Source:
Escherichia Coli.

Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser.

Purity:
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility:
It is recommended to reconstitute the lyophilized Flt3-L in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Formulation:
The protein was lyophilized with no additives.

Stability:
Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution Flt3-Ligand should be stored at 4 °C between 2-7 days and for future use below -18 °C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Activity:
The ED50 range=0.5-1.0 ng/ml corresponding to a specific activity of 1-2MUnits/mg, calculated by the dose-dependant stimulation of the proliferation of human OCMI-AML5 cells.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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