- Description
- Specifications
Catalog #: ENZ-352
Description:
E.Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases).
Synonyms:
Uracil DNA Glycosilase, Uracil DNA Glycosylase, UNG.
Source:
Escherichia Coli strain that carries the UNG gene from E.coli.
Applications:
Glycosilase mediated single nucleotide polymorphism detection (GMPD).
Site-directed mutagenesis.
As a probe for protein-DNA interaction studies.
Rapid and efficient cloning of PCR products.
Elimination carry-over contamination in PCR.
Formulation:
UNG solution in 10mM Tris-Hcl (pH-7.4 at 25 °C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol.
Stability:
Uracil DNA Glycosilase although stable at 15 °C for 1 week, should be stored desiccated below -18 °C.
Please prevent freeze-thaw cycles.
Activity:
The Specific Activity was found to be 5U/ µl.
Physical Appearance:
Sterile Filtered colorless solution.
Reaction Conditions:
1X UNG Reaction Buffer, incubate at 37 °C.
UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.
10X UNG Reaction Buffer:
200mM Tris-HCl (pH 8.0 at 25 °C), 10mM DTT and 10mM EDTA.
Inhibition and Inactivation:
Inactivated by heating at 95 °C for 10min. Enzye activity is partially restored at temperatures lower than 55 °C.
Unit Definition:
1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37 °C.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
