- Description
- Specifications
Catalog #: HOR-250
Description:
Human Chorionic Gonadotropin is produced from a sterile preparation of placental glucoprotein urine of pregnant women having a total molecular mass of 36,700 Dalton. The hCG consists of 237 amino acids, a chain-92 amino acids and b chain-145 amino acids. The hCG is purified by proprietary chromatographic techniques.
Synonyms:
Chorionic gonadotropin, hCG, CG.
Source:
Urine of pregnant women.
Purity:
Purity is 98%.
Free of: HbsAg, Hepatitis B surface antigen and antibodies to HIV, Hepatitis C and HCV.
Solubility:
It is recommended to reconstitute the lyophilized Human Chorionic Gonadotropin in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The hCG was lyophilized with no additives.
Stability:
Lyophilized hCG although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution CG-beta should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The activity was found to be 6767IU/mg.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.06 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of hCG as a Reference Standard.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
Title: Human Chorionic Gonadotropin Modulates Prostate Cancer Cell Survival after Irradiation or HMG CoA Reductase Inhibitor Treatment
Publication: Published online before print October 18, 2006, doi: 10.1124/mol.106.031153 Molecular Pharmacology January 2007 vol. 71 no. 1 259-275
Link:http://molpharm.aspetjournals.org/content/71/1/259.full
