- Description
- Specifications
Catalog #: HIV-124
Description:
The E.coli derived 20 kDa recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 nef immunodominant regions, 3-190 amino acids.
The HIV-1 nef is fused to beta-galactosidase (114 kDa) at the N-terminus.
Source:
Escherichia Coli.
Amino Acid Sequence:
kwskssv igwptvrerm rraepaadgv gaasqdlekh gaitssntaa tnadcawlea
qeeeevgfpv tpqvplrpmt ykaavdlshf lkekgglegl ihsqrrqdil dlwiyhtqgy
fpdwqnytpg pgirypltfg wcyklvpvep ekleeankge ntsllhpvsl hgmddperev
lewrfdsrla.
Applications:
HIV-1 nef antigen in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
Purity:
Greater than 95.0% as determined by HPLC analysis and SDS-PAGE.
Formulation:
HIV-1 nef protein solution in 8M urea, 20mM Tris-HCl pH 8.0, 10mM b-mercaptoethanol.
Stability:
HIV-1 nef although stable at 4 °C for 1 week, should be stored below -18 °C.
Please prevent freeze thaw cycles.
Specificity:
HIV-1 nef is immunoreactive with all sera of HIV-1 infected individuals.
Physical Appearance:
Sterile filtered colorless clear solution.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.