- Description
- Specifications
Catalog #: HIV-107
Description:
The E.Coli derived recombinant protein contains the C- terminus of gp46 and most of p21E of HTLV-1. This non-fusion, E. coli-derived protein, starts from HIV-1 env. Amino acid 165, and is ending in amino acid 440, Mw 27 kDa.
Applications:
HTLV-1 Envelope can be used as an antigen in ELISA and Western Blots. Excellent reagent for correct detection of HTLV infections, with minimal specificity problems.
Purification Method:
HTLV-1 Envelope was purified by proprietary chromatographic technique.
Purity:
HTLV-1 Envelope protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.
Solubility:
It is recommended to reconstitute the lyophilized HTLV-1 Envelope in sterile 18M?-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
10mM Na-PO4 pH 6.0, 0.1% SDS and 1mM DTT.
Stability:
Lyophilized HTLV-1 Envelope although stable at room temperature for 3 weeks, should be stored desiccated below - 18 °C. Upon reconstitution HTLV-1 Envelope should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Specificity:
Immunoreactive with all sera of HTLV-I and HTLV-II infected individuals with antibody response to HTLV envelope.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
