- Description
- Specifications
Catalog #: CHM-334
Description:
I-TAC Human Recombinant (Interferon-inducible T-cell alpha chemoattractant) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 8300 Dalton.
The I-TAC is purified by proprietary chromatographic techniques.
Synonyms:
Small inducible cytokine B11, CXCL11, Interferon-inducible T-cell alpha chemoattractant, I-TAC, Interferon-gamma-inducible protein 9, IP-9, H174, Beta-R1, chemokine (C-X-C motif) ligand 11, IP9, b-R1, SCYB11, SCYB9B, MGC102770.
Source:
Escherichia Coli.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids of I-TAC was determined and found to be, Phe-Pro-Met-Phe-Lys.
Purity:
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized I-TAC in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The I-TAC protein was lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Stability:
Lyophilized I-TAC although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution I-TAC should be stored at 4 °C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
ED50 range=1-10ng/ml corresponding to a Specific Activity of 10,000-100,000IU/mg determined by the dose dependent chemotaxis of human lymphocytes cultured in the presence of IL-2.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
