- Description
- Specifications
Catalog #: CYT-464
Description:
IGFBP5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 253 amino acids and having a molecular mass of 28613 Dalton.
IGFBP5 is purified by proprietary chromatographic techniques.
Synonyms:
IGFBP-5, IBP-5, IGF-binding protein 5.
Source:
Escherichia Coli.
Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Gly-Ser-Phe.
Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-5 in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
IBP-5 was lyophilized from a concentrated (1mg/ml) solution containing 10mM sodium Citrate PH 3.0.
Stability:
Lyophilized IBP5 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IGFBP 5 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50, calculated by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 0.3 µg/ml in the presence of 15ng/ml of Human IGF-II.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Usage:
ProsSec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
References:
Title:Effects of Dietary Carbohydrate Modification
in Persons with the Metabolic Syndrome
- A Transcriptomics Approach in Adipose Tissue
Publication:Department of Clinical Nutrition, Food and Health Research Centre
Institute of Public Health and Clinical Nutrition
Faculty of Health Sciences
University of Eastern Finland Kuopio 2010
Link:http://epublications.uef.fi/pub/urn_isbn_978-952-61-0024-1/urn_isbn_978-952-61-0024-1.pdf
