- Description
- Specifications
Catalog #: CYT-216
Description:
Insulin-Like Growth Factor-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7655 Dalton.
IGF-I is purified by proprietary chromatographic techniques.
Synonyms:
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.
Source:
Escherichia Coli.
Amino Acid Sequence:
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA.
Purity:
Greater than 95.0% as determined by
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE.
Solubility:
It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MΩ–cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Formulation:
The protein was lyophilized with no additives.
Stability:
Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution IGF1 should be stored at 4 °C between 2-7 days and for future use below -18 °C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Activity:
The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is < 1.0ng/ml, corresponding to a specific activity of 1,000,000U/mg. For most in-vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20 ng/ml.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard.
Usage:
Denovo Biotechnology's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
References:
1.Title:Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis.
Publication: Journal of Endocrinology (2009) 202, 287–297.
Link: http://joe.endocrinology-journals.org/content/202/2/287.full.pdf
Application: support spermatogenesis in an androgen-independent manner (Leal et al. 2006)
2.Title:Suppression of Anoikis by SKP2 Amplification and Overexpression Promotes Metastasis of Esophageal Squamous Cell Carcinoma .
Publication:doi: 10.1158/1541-7786.MCR-08-0092 Mol Cancer Res January 2009 7; 12
Link:http://mcr.aacrjournals.org/content/7/1/12.full
3.Title:MECHANISMS OF BIOMATERIAL MEDIATED FIBROTIC RESPONSES AND STRATEGIES TO
IMPROVE TISSUE REACTIONS TO BIOMATERIAL IMPLANTS.
Publication:THE UNIVERSITY OF TEXAS AT ARLINGTON
May 2010
Link:http://dspace.uta.edu/bitstream/handle/10106/4918/Thevenot_uta_2502Dx10646.pdf?sequence=1
